G2150
L-Glutamine solution
200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma
Synonym(s):
L-Glutamine for cell culture
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
Recommended Products
grade
Hybri-Max™
Quality Level
sterility
sterile-filtered
Assay
≥99% (TLC)
form
solution
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
color
clear colorless
shipped in
dry ice
storage temp.
−20°C
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
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General description
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Application
The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
Preparation Note
Prepared in cell culture grade water.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Application
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Chromatograms
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