F5387
Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Mouse IgG (Fc specific)–Fluorescein isothiocyanate
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About This Item
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biological source
goat
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
storage condition
protect from light
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
indirect immunofluorescence: 1:32
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Specificity
Goat polyclonal anti-Mouse IgG (Fc specific)–FITC antibody detects all mouse IgG subclasses, it shows no reactivity with mouse IgG Fab fragment or human IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum result in single arcs of precipitation. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.
Immunogen
Purified mouse IgG Fc fragment
Application
Anti-Mouse IgG (Fc specific)-FITC antibody has be used for
- Immunofluorescence
- Immunohistochemistry
- Immunostaining
- Confocal microscopy
- Flow cytometry
Biochem/physiol Actions
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections IgG is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.
Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.
Other Notes
Antibody adsorbed with human IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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