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Key Documents

AFRC 58D6

Reactivity: Anti-Haemagglutinin, influenza, 94120827

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About This Item

UNSPSC Code:
41106514

biological source

Horse x Mouse/Horse Hybridoma

ImmunoDon

Horse sub-mandibular lymph node

Myeloma

AFRC 17D1

Reactivity

Anti-Haemagglutinin, influenza

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Horse sub-mandibular lymph node x AFRC 17D1

Cell Line Description

The heterohybridoma AFRC 58D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 58D6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Immunogen

Influenza A equine 2 virus

Application

Haemagglutinin detection
The isotype of the monoclonal antibody produced by this cell line is horse IgG.

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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