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Key Documents

10021102

Bob cell line human

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About This Item

UNSPSC Code:
41106514

biological source

human prostate

form

liquid

growth mode

Adherent

karyotype

4n (81 to 94)

morphology

Cobblestone

products

10% 3-D sphere formation in Matrigel. Migratory and invasive when compared to benign prostate tumour.

receptors

CD44, alpha2 integrin, alpha6 integrin, EGFR, FGFR1, LIFR

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human prostate cancer spontaneously immortalized, serum-free

Cell Line Description

First spontaneously immortalized prostate cancer cell line to be established from a trans-rectal needle biopsy (TRBP) of a patient with castration-resistant prostate cancer (CRPC). Bob is a novel pre-clinical model for functional studies in CRPC and especially for studying the CRPC "basal" phenotype. This cell line is cultured in serum-free culture medium, a derivative of Bob is also available i.e. SerBob, Sigma Catalogue number 10021101, which has been adapted to medium containing serum. SerBob is more differentiated and invasive than Bob. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Application

For complete product information, please see ECACC
Prostate cancer research including drug testing.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,14
D16S539: 10,12
D5S818: 13
D7S820: 10,11
THO1: 9
TPOX: 8,10
vWA: 17

Culture Medium

Keratinocyte-SFM supplied with prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53), bovine pituitary extract (BPE) and glutamine) + 2ng/ml leukaemia inhibitory factor, 2ng/ml stem cell factor + 100ng/ml cholera toxin + 1ng/ml granulocyte macrophage colony stimulating factor.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2-4 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells must be centrifuged at 400 x g for 5 minutes to remove the trypsin at each subculture. Cells are cryopreserved in 45% conditioned media: 45% fresh media: 10% DMSO. At confluence 5-7 x 104 cells/cm2 can be expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Gerhardt Attard et al.
The Prostate, 69(14), 1507-1520 (2009-06-23)
New in vitro models of castration-resistant prostate cancer (CRPC) are urgently required. Trans-rectal needle biopsies (TRBP) of the prostate were performed for research purposes on progressing CRPC patients who had not received prior treatment to the prostate. Biopsies were immediately

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