HPLC Analysis of Nucleosides on Ascentis^® Phenyl

column

Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 μm particles (581613-U)

mobile phase

10 mM ammonium formate (pH 3.0 with formic acid)

flow rate

0.2 mL/min

column temp.

35 °C

detector

UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell

injection

1 μL

sample

as indicated, in mobile phase