跳转至内容

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

摘要

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

材料

货号

品牌

产品描述

Sigma-Aldrich

二甲基亚砜, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sigma-Aldrich

二甲基亚砜, for molecular biology

Sigma-Aldrich

二甲基亚砜, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Sigma-Aldrich

二甲基亚砜, ≥99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥99% (HPLC), from Saccharomyces cerevisiae

Sigma-Aldrich

盐酸溶液, 1.0 N, BioReagent, suitable for cell culture

Sigma-Aldrich

二甲基亚砜, anhydrous, ≥99.9%

Sigma-Aldrich

二氯甲烷, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

Sigma-Aldrich

氯化钙溶液, BioUltra, for molecular biology, ~1 M in H₂O

Sigma-Aldrich

盐酸, 36.5-38.0%, BioReagent, for molecular biology

Sigma-Aldrich

氯化钙, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

D-(+)-海藻糖二水合物, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

甲醇, anhydrous, 99.8%

Sigma-Aldrich

N-琥珀酰-丙酰氨-丙酰氨-脯酰氨-苯丙氨酸对硝基酰苯胺

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥98.5% (HPLC), from Saccharomyces cerevisiae

Sigma-Aldrich

D-(+)-海藻糖二水合物, ≥99% (HPLC), from starch

Sigma-Aldrich

二甲基亚砜, BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺, ≥99%

Supelco

盐酸溶液, volumetric, 0.1 M HCl (0.1N), endotoxin free

Sigma-Aldrich

盐酸, SAJ first grade, 35.0-37.0%

Sigma-Aldrich

氧化铝, nanopowder, <50 nm particle size (TEM)

Sigma-Aldrich

脂肪酶丙烯酸树脂, ≥5,000 U/g, recombinant, expressed in Aspergillus niger

Sigma-Aldrich

甲醇, JIS special grade, ≥99.8%

Sigma-Aldrich

乙酸乙酯, anhydrous, 99.8%

Sigma-Aldrich

氯化钙, anhydrous, powder, 99.99% trace metals basis

Sigma-Aldrich

氯化氢, ReagentPlus^®, ≥99%

Sigma-Aldrich

盐酸, JIS special grade, 35.0-37.0%

Sigma-Aldrich

盐酸溶液, 1 M

Sigma-Aldrich

α-糜蛋白酶来源于牛胰腺, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

Sigma-Aldrich

丙酮, JIS special grade, ≥99.5%