跳转至内容
Merck
  • Development of a new high-performance liquid chromatography method with diode array and electrospray ionization-mass spectrometry detection for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L.

Development of a new high-performance liquid chromatography method with diode array and electrospray ionization-mass spectrometry detection for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L.

Journal of chromatography. A (2014-05-27)
Francesco Pio Prencipe, Virginia Brighenti, Margherita Rodolfi, Andrea Mongelli, Chiara dall'Asta, Tommaso Ganino, Renato Bruni, Federica Pellati
摘要

The study was aimed at developing a new analytical method for the metabolite fingerprinting of bioactive compounds in Humulus lupulus L. (hop), together with a simple extraction procedure. Different extraction techniques, including maceration, heat reflux extraction (HRE), ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE), were compared in order to obtain a high yield of the target analytes. Dynamic maceration for 30min with MeOH-HCOOH (99:1, v/v) as the extraction solvent provided the best result in terms of recovery of secondary metabolites. The analysis of hop constituents, including prenylflavonoids and prenylphloroglucinols (bitter acids), was carried out by means of HPLC-UV/DAD, HPLC-ESI-MS and MS(2), using an ion trap mass analyzer. An Ascentis Express C18 column (150mm×3.0mm I.D., 2.7μm) was used for the HPLC analysis, with a mobile phase composed of 0.25% formic acid in both water and acetonitrile, under gradient elution. The method validation was performed to show compliance with ICH guidelines. The validated technique was successfully applied to the phytochemical analysis of ten commercial cultivars and twenty-three wild Italian hop genotypes, thus demonstrating to be a reliable and useful tool for the comprehensive multi-component analysis of hop secondary metabolites.

材料
货号
品牌
产品描述

Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm
Sigma-Aldrich
间苯三酚, ≥99.0% (HPLC)
Supelco
Ascentis® Express C18, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Ascentis® Express C18,5μm HPLC色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 7.5 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 10 cm × 3 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 15 cm × 3 mm
Supelco
Ascentis® Express C18,2.7um 保护柱芯, 2.7 μm particle size, L × I.D. 5 mm × 3 mm, pkg of 3 ea
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Ascentis® Express C18,5μm HPLC色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Ascentis® Express C18 PCP HPLC Columns, 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Ascentis® Express C18,2.7um 保护柱芯, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea
Supelco
Ascentis® Express C18,2.7um 保护柱芯, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Ascentis® Express C18, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Ascentis® Express C18, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 5 cm × 2.1 mm
Sigma-Aldrich
间苯三酚, suitable for plant cell culture, BioReagent
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 3 cm × 2.1 mm
Supelco
Ascentis® Express C18,5μm HPLC色谱柱, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 7.5 cm × 2.1 mm
Supelco
Ascentis® Express C18, 2 μm Guard Cartridge, 2 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea
Supelco
Ascentis® Express C18,5μm HPLC色谱柱, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Ascentis® Express C18 PCP HPLC Columns, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Ascentis® Express C18,5μm HPLC色谱柱, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Ascentis® Express C18, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 3 mm
Supelco
Ascentis® Express C18, 5 μm Guard Cartridge, 5 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea