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Merck
  • Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes.

Synthesis and use of QCy7-derived modular probes for the detection and imaging of biologically relevant analytes.

Nature protocols (2013-12-07)
Orit Redy-Keisar, Einat Kisin-Finfer, Shiran Ferber, Ronit Satchi-Fainaro, Doron Shabat
摘要

This protocol describes the synthesis of modular turn-ON QCy7-based probes for the detection of biologically relevant analytes, such as hydrogen peroxide, ubiquitous sulfhydryl and β-galactosidase. The probes presented herein are prepared through a simple procedure that involves the preliminary alkylation of 4-hydroxy-isophthalaldehyde with a relevant analyte-responsive protecting group, followed by condensation of the resulting product with 2 equivalents of sulfo-indolium moieties. Evaluation of the turn-ON near-IR fluorescence response to their relevant analytes for the three different QCy7 probes is also reported. The preparation of a QCy7 diagnostic probe requires 1-2 d. Probes for other analytes can be prepared according to this modular procedure by incorporating a specific analyte-responsive group as a triggering substrate.

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Sigma-Aldrich
L-半胱氨酸 盐酸盐, anhydrous, ≥99.0% (RT)
Sigma-Aldrich
β 半乳糖苷酶 来源于大肠杆菌, Grade VI, lyophilized powder, ≥250 units/mg protein