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  • Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.

Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.

Nature methods (2020-05-20)
Taeyun Ku, Webster Guan, Nicholas B Evans, Chang Ho Sohn, Alexandre Albanese, Joon-Goon Kim, Matthew P Frosch, Kwanghun Chung
摘要

We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.

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丙烯酰胺, for molecular biology, ≥99% (HPLC)
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硫酸葡聚糖 钠盐 来源于明串珠菌 属, for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer
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1,2-二甲氧基乙烷, suitable for HPLC, 99.9%, inhibitor-free
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Eosin Y-solution 0.5% alcoholic, for microscopy