研究用螯合和还原剂
研究中的螯合剂
螯合剂可用作络合剂。它们有助于各种化学和生物化学分析的进行,可用作缓冲液成分、核酸/蛋白质分解抑制剂、酶反应催化剂,用于亲和色谱和细胞培养。螯合剂是强效治疗剂,可平衡代谢和相关疾病,如贫血、癌症、急性肾病、衰老、神经退行性疾病等。在工业界、分析和实验室实验中,这些金属离子螯合剂可用作萃取剂、指示剂和金属分离剂。
研究中的还原剂
还原剂可用于蛋白组学方法,如蛋白质纯化、变性和增溶,并且特别擅长于稳定游离巯基并还原蛋白质和肽中的二硫键(SH)。在蛋白质纯化中,它们可作为缓冲液中酶的稳定剂,防止半胱氨酸残基氧化导致的聚集。还原剂也可作为生物抗氧化剂,防止蛋白质氧化并使核糖核酸酶变性。研究表明螯合剂和还原剂的联合使用有助于去除阳离子重金属和阴离子污染物,如砷。
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