推荐产品
product name
Ascentis® Si HPLC 色谱柱, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
材料
stainless steel column
品質等級
agency
suitable for USP L3
產品線
Ascentis®
特點
endcapped: no
製造商／商標名
Ascentis®
包裝
1 ea of
參數
≤70 °C temp. range
400 bar pressure (5801 psi)
技術
HPLC: suitable
LC/MS: suitable
長度 × 內徑
10 cm × 2.1 mm
表面積
450 m2/g
雜質
<5 ppm metals
基質
fully porous particle
silica gel high purity, spherical
基質活性組
silica phase
粒徑
5 μm
孔徑
100 Å
工作pH值範圍
2-6
應用
food and beverages
分離技術
hydrophilic interaction (HILIC)
normal phase
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一般說明
Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。
Ascentis Si 是具有高载样量和优良峰形的硅胶柱。Ascentis Si 在正相和 HILIC/ANP（水性正相）模式下都可使用。
Ascentis Si 是具有高载样量和优良峰形的硅胶柱。Ascentis Si 在正相和 HILIC/ANP（水性正相）模式下都可使用。
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
实验方案
氨基酸分析是精确测定蛋白质量的合适工具，但也提供有关相对氨基的详细信息。
Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.
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