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Key Documents

ZRB1639

Sigma-Aldrich

Anti-TrkA Antibody, clone 2J4 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

别名:

High affinity nerve growth factor receptor;EC:2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;gp140trk;p140-TrkA

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

品質等級

重組細胞

expressed in HEK 293 cells

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

2J4, recombinant monoclonal

描述

recombinant, expressed in HEK 293 cells

產品線

ZooMAb® learn more

形狀

lyophilized

分子量

calculated mol wt 87.5 kDa
observed mol wt ~140 kDa

純化經由

using Protein A

物種活性

human

物種活性(以同源性預測)

monkey

包裝

antibody small pack of 25 μL

加強驗證

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技術

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG

表位序列

C-terminal

Protein ID登錄號

UniProt登錄號

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... NTRK1(4914)

一般說明

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2J4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects TrkA . It targets an epitope within 21 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal, cytoplasmic domain of human TrkA.

應用

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TrkA in K562 cell lysate.

Tested Applications

Western Blotting Analysis (WB): 1:10,000 dilution of this antibody detected His-tagged recombinant fragment of Human TrkA.

Affinity Binding Assay: A representative lot of this antibody bound TrkA peptide with a KD of 4.3 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TrkA in SH-SY5Y cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TrkA in human adrenal gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

High affinity nerve growth factor receptor (UniProt: P04629; also known as EC:2.7.10.1, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, Trk-A, gp140trk, p140-TrkA) is encoded by the NTRK1 (also known as MTC, TRK, TRKA) gene (Gene ID: 4914) in human. TrkA is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-32), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 33-423), a transmembrane domain (aa 424-439), and a cytoplasmic domain (aa 440-796). The extracellular domain of Trk-A is composed by five domains: a leucine-rich region, two cysteine-rich domains and two immunoglobulin (Ig)-like domains. TrkA serves as a receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous system through regulation of proliferation, differentiation, and survival of sympathetic and nervous neurons. It serves as a high affinity receptor for NGF, which is its primary ligand. It can also bind and be activated by neurotrophin-3 (NTF3). However, NTF3 only supports axonal extension through NTRK1 but does not affect neuron survival. Upon dimeric NGF ligand-binding, it undergoes homodimerization, autophosphorylation. and activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wehrman, T., et al. (2007). Neuron. 53(1); 25-38; Covaceuszach, S., et al. (2005). Proteins. 58(3); :717-727; Jing, S., et al. (1992). Neuron. 9(6); 1067-1079).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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