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應用
酵母氨基酸缺陷型合成培养基添加剂不含尿嘧啶、亮氨酸和色氨酸，可用于筛查：
- 携带pCRISPRyl_URA3sgRNA 质粒的 PO1f 菌株的转化株
- 解脂耶氏酵母 PO1f 菌株的阳性转化株
- CRISPR和 RNA辅助体内定向进化（CRAIDE）突变体
由于在缺乏特定培养基成分（比如氨基酸、嘌呤或嘧啶）的情况下无法生长，营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化，可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基，可以提高产量和生长速度，并增加筛选文库或进行基因敲除时转化的成功率。
其他說明
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
準備報告
每升培养基溶解10.4克添加剂。
相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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