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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
包裝
antibody small pack of 25 μL
技術
dot blot: 1:5,000 using purified recombinant thioredoxin
western blot: 1:5,000 using E. coli extract
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
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一般說明
The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin from E. coli consists of a single polypeptide chain of 108 amino acids with a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.
特異性
Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.
免疫原
recombinant E. coli thioredoxin.
應用
Anti-Thioredoxin antibody produced in rabbit has been used in:
- immunohistochemistry
- immunoblotting
- dot blot immunoassay
- ouchterlony double diffusion
- immunodetection
- western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.
生化／生理作用
Thioredoxin is a small electron transport protein that serves as the hydrogen donor in the enzymatic reduction of ribonucleotides to deoxyribonucleotides. The thioredoxin system is involved in other reductive processes such as the enzymatic reduction of methionine sulfoxide and sulfate. The oxidation-reduction function of thioredoxin is linked to a single intra-molecular disulfide bridge, forming a 14 member ring. The system is particularly useful for high level production of soluble fusion proteins in the E. coli cytoplasm. In many cases, these fusion proteins fold correctly and thus display full biological activity.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
The thioredoxin system in cancer
Arner ESJ and Holmgren A
Seminars in Cancer Biology, 16(6), 420-426 (2006)
Wouter S P Jong et al.
Microbial cell factories, 16(1), 50-50 (2017-03-23)
Heterologous protein production in Escherichia coli often suffers from bottlenecks such as proteolytic degradation, complex purification procedures and toxicity towards the expression host. Production of proteins in an insoluble form in inclusion bodies (IBs) can alleviate these problems. Unfortunately, the
p23 protects the human aryl hydrocarbon receptor from degradation via a heat shock protein 90-independent mechanism
Pappas B, et al.
Biochemical Pharmacology, 152(1), 34-44 (2018)
Chickpea ferritin CaFer1 participates in oxidative stress response, and promotes growth and development
Chakraborty S, et al.
Scientific Reports (2016)
The anti-tumor effects of calorie restriction are correlated with reduced oxidative stress in ENU-induced gliomas
Mahlke MA, et al
Pathobiology of aging & age related diseases, 1(1), 7189-7189 (2011)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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