跳转至内容
Merck

SAB4200735

Sigma-Aldrich

Anti-CRISPR/CAS9 -FITC antibody, Mouse monoclonal

clone 7A9-3A3, purified from hybridoma cell culture

别名:

Anti-CRISPR-associated protein-9 nuclease, Anti-Crispr, Anti-Crispr RNA

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

共軛

FITC conjugate

品質等級

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

7A9-3A3, monoclonal

形狀

buffered aqueous solution

儲存條件

protect from light

濃度

~1.0 mg/mL

技術

immunofluorescence: 1:200-1:400 using human HEK-293T cells over-expressing CAS9 protein

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) belongs to the type II CRISPR/CAS9 system. It is part of an adaptive immune system of the Streptococcus pyogenes SF370, protecting from pathogens′ target genes by cleaving the foreign DNA in a sequence-dependent manner.
The type II CRISPR/Cas system has been adapted to expression in eukaryotic cells. The Cas9 endonuclease can be engineered with a single gRNA (guide RNA), directing a DNA double-strand break (DSB) at a desired genomic location. As a result, the cell activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR). In comparison to other genome-editing technologies such as designer zinc fingers (ZFs), transcription activator–like effectors (TALEs) and homing meganucleases, the CRISPR/CAS9 system is a scalable, affordable and easy to engineer. Therefore, the anti-CRISPR/CAS9-FITC conjugated antibody can be a useful tool for detecting CRISPR/CAS9 positively transfected cells, revealing DSB sites in their genome.

免疫原

Recombinant protein within the N-terminal region of Streptococcus pyogene Cas9

應用

Monoclonal Anti-CRISPR/CAS9-FITC recognizes the CAS9 protein in CAS9 construct over-expression systems. The antibody may be used in various immunochemical techniques including Immunofluorescence. The antibody successfully recognizes mutant Cas9 variants, including nickase Cas9 and dead Cas9 (dCas9) and does not cross react with FnCas9 from Francisella novicida bacteria and Cpf1 proteins from Acidaminococcus sp. (strain BV3L6) and Lachnospiraceae bacterium ND2006.

外觀

Solution in 0.01M phosphate buffered saline pH 7.4 containing 1% bovine serum albumin and 15mM sodium azide.

儲存和穩定性

For continuous use, store at 2-8°C, protected from light for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

其他說明

This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门