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Key Documents

SAB3700420

Sigma-Aldrich

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

别名:

Anti-Pig IgG F(ab′)2-Peroxidase antibody produced in rabbit, Anti-Porcine IgG F(ab′)2-Peroxidase antibody produced in rabbit, HRP

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

rabbit

共軛

peroxidase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

lyophilized powder

物種活性

swine

技術

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

Immunoglobulin G (IgG) is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains.

特異性

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Swine IgG F(ab′)2 and Swine Serum. No reaction was seen against Swine IgG F(c) .

免疫原

Swine IgG F(ab′)2 fragment

應用

Anti-Swine IgG F(ab′)2-Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA).(3)

物理性質

Antibody format: IgG

外觀

Supplied in 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

重構

Reconstitute with 1.0 mL deionized water (or equivalent).

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

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訊號詞

Warning

危險聲明

防範說明

危險分類

Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Antibody structure, instability, and formulation
Immunobiology: The Immune System in Health and Disease, 96(1), 1-26 (2001)
Lycopene affects the immune responses of finishing pigs
Marcelise RF, et al.
Italian Journal of Animal Science, 17(3), 666-674 (2018)

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