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Merck

P6097

Sigma-Aldrich

预制琼脂糖凝胶

24 wells (2 × 12-well portrait orientation)

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About This Item

分類程式碼代碼:
41105300
NACRES:
NB.22

等級

for molecular biology

形狀

gel form

成份

儲存溫度

room temp

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一般說明

Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide. RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0) Quality

應用

预制琼脂糖凝胶设计用于高分辨分离核酸,获得低背景荧光的锐利DNA和RNA条带。 预制胶用铝箔独立密封在塑料托盘中,可避免来自设备和操作的污染。 凝胶可在塑料托盘中直接开始电泳。

TBE-琼脂糖凝胶提供两种常用浓度用于DNA电泳:1%和4%。 TBE-琼脂糖凝胶中含有溴化乙锭(0.5 μg/ml),显色方便。 TBE-琼脂糖凝胶提供8、20和24孔规格。 1.0%凝胶的分离范围是500-1000 bp,4%凝胶为8-1000 bp。

MOPS - 琼脂糖凝胶(1.25%)配方不含变性剂(如甲醛或乙二醛),可用于分析总RNA、体外RNA转录本和Northern印迹。 RNA电泳一般不干扰染色。但是如果怀疑RNA有明显的二级结构,则应从产品插页中选择一种方法使RNA变性。 1.25% MOPS凝胶的分离范围是0.25-10 kb。
Suitable for separation of DNA fragments ranging from 8 – 1000 base pairs.

包裝

20 个铝箔独立包裹的凝胶。

成分

Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.

其他說明

DNA 琼脂糖凝胶含有 1×TBE 缓冲液(0.089 MTris-硼酸盐,pH 8.3,2 mM EDTA)和 0.5 mg/mL 溴化乙锭。
RNA 琼脂糖凝胶含 1×MOPS(20 mM MOPS,5 mM 乙酸钠,1 mM EDTA 和 1 mM EGTA,pH 7.0)

品質

未检出 DNase 或 RNase 活性

外觀

凝胶尺寸: 6.0 × 9.5 cm,5.5 mm 厚
托盘尺寸: 6.8 × 10.2 cm 容器托盘;适合普通水平电泳装置
加样孔: 8 孔(纵向)、20 孔(横向)、24 孔(2 × 12 孔纵向)

相關產品

产品编号
说明
价格

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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实验方案

These gels are suitable for separating nucleic acids, giving sharp DNA bands and low background fluorescence.

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