推荐产品
包裝
pkg of 1 ea
製造商/商標名
Cytiva 28-9505-17
參數
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
床尺寸
7.7 mm × 100 mm
床體積
4.7 mL
柱內徑
7.7 mm
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning
2-14(Medium uncharged with metal ions.)
工作範圍
3-12
容量
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
適合性
suitable for bioprocess medium
一般說明
應用
IMAC Sepharose™ 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.
The medium is available prepacked in HiTrap, HiScreen, and HiPrep™ column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep™ IMAC FF 16/10 columns. Prepacked HiScreen™ IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.
特點和優勢
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal ion of choice.
- Optimal bead size for scale-up.
- High binding capacity.
- Prepacked HiScreen™ columns for method optimization and process development.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
儲存和穩定性
分析報告
法律資訊
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
实验方案
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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