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Key Documents

GE17-1180-01

Resource™ S

Cytiva 17-1180-01, pack of 6 mL

别名:

Resource S Column

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About This Item

分類程式碼代碼:
47101511
NACRES:
NA.56

ligand

sulfonate group

描述

Ion Exchanger Type (value)

包裝

pack of 6 mL

製造商／商標名

Cytiva 17-1180-01

參數

87 psi

床尺寸

16 mm × 30 mm

床體積

6 mL

基質

polystyrene/divinylbenzene

樣品平均尺寸

15 μm

粒徑

15-15 μm

cleaning

1-14

工作範圍

2-13

適合性

suitable for bioprocess medium (BioProcess medium. Columns not suitable for use with ÄKTAprime plus system.)
suitable for bioprocess medium

一般說明

RESOURCE™ S columns are prepacked with SOURCE™ 15S, a strong cation exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

特點和優勢

  • SOURCE™ 15Q and 15S media are well suited for fast, high-resolution purifications and easy scale-up.
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

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商品

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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实验方案

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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