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Key Documents

GE17-0719-10

CM Sepharose™ Fast Flow

Cytiva 17-0719-10, pack of 25 mL

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

carboxymethyl

描述

Ion Exchanger Type (value)

包裝

pack of 25 mL

製造商／商標名

Cytiva 17-0719-10

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning in place

2-14

工作範圍

4-13

適合性

suitable for bioprocess medium

相关类别

一般說明

CM Sepharose™ Fast FLow is a weak cation exchanger based on the well established Sepharose™ Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

應用

CM Sepharose™ Fast FLow is part of the Sepharose™ Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with carboxymethyl (CM) weak cation exchange groups. CM Sepharose™ Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with CM Sepharose™ Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, CM Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Well-proven weak cation exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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