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Merck

GE17-0402-03

HiTrap® Protein A High Performance

Cytiva 17-0402-03, pack of 2 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

native protein A

包裝

pack of 2 × 1 mL

製造商/商標名

Cytiva 17-0402-03

儲存條件

(20% Ethanol)

參數

42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

尺寸

樣品平均尺寸

34 μm

cleaning

2-10

工作範圍

3-9

儲存溫度

2-8°C

一般說明

HiTrap® protein A HP is a prepacked protein A Sepharose High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

應用

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

特點和優勢

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as ÄKTA® design or FPLC System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

实验方案

General instructions for affinity purification using HiTrap columns from Cytiva.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

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