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G3776

Sigma-Aldrich

鸟苷5′-三磷酸 钠盐 溶液

HPLC purified, aqueous solution for RNA polymerase transcription

别名:

GTP

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About This Item

CAS号:
36051-31-7
MDL號碼:
MFCD00077781
分類程式碼代碼:
41106305
PubChem物質ID:
24895138
NACRES:
NA.52

生物源

Porcine brain
bacterial (Corynebacterium)
yeast

化驗

≥95% (HPLC)

形狀

liquid

濃度

100 mM in H2O (adjusted to pH 7)

顏色

colorless

異物活動

DNase, RNase and Nickase, none detected

運輸包裝

dry ice

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1

InChI 密鑰

KZRMTEVIDYXWQW-CYCLDIHTSA-K

基因資訊

human ... HRAS(3265)

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應用

5′-三磷酸鸟苷钠盐溶液已用于：

  • 在转导蛋白的纯化过程中重构转导蛋白。
  • 作为 3.3X 负载缓冲液的组分，组装 32P-miR-31—3xFLAG-AGO2 复合物
  • 作为核苷酸底物混合物的组分，以评估 MutT 同源物 1（MTH1）对甲基化核苷三磷酸盐的水解活性
  • DNA 依赖性 RNA 聚合酶转录

生化／生理作用

5′-三磷酸鸟苷（GTP）占总核苷酸三磷酸池的近 25%，是一种核苷酸阴离子。它参与多种生物功能，如通过激活 GTP 结合蛋白、RNA 和蛋白质合成的细胞信号传导，以及产生二级信使环鸟苷一磷酸（cGMP）。GTP 还参与了严格的信号素的生产。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type N95 (US)

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Giselle Cerchiaro et al.
Redox report : communications in free radical research, 14(2), 82-92 (2009-04-25)
Levels of oxidized guanosine base in DNA have become a hallmark biomarker in assessing oxidative stress implicated in a variety of disease and toxin-induced states. However, there is evidence that the guanosine in the nucleotide triphosphate pool (GTP) is more
Reyad A Elbarbary et al.
Methods (San Diego, Calif.), 152, 18-22 (2018-05-20)
MicroRNAs (miRNAs) comprise a class of small non-coding RNAs that regulate the stability and/or translatability of most protein-coding transcripts. Steady-state levels of mature miRNAs can be controlled through mechanisms that influence their biogenesis and/or decay rates. Pathways that mediate mature
In vitro biochemical assays to monitor rhodopsin function.
Sammons J and Gross AK
Methods in Molecular Biology, 884, 167-181 (2012)
Water-soluble luminescent copper nanoclusters reduced and protected by histidine for sensing of guanosine 5'-triphosphate
Zhao X J and Huang, C Z
New. J. Chem., 38(8), 3673-3677 (2014)
Fu-Jung Chang et al.
The Journal of biological chemistry, 280(12), 11513-11519 (2005-01-15)
The mechanism of desensitization of the nitric oxide (NO) receptor (alpha1.beta1 isoform of soluble guanylyl cyclase, sGC) is not known. Models of the structure of alpha1.beta1, based on the x-ray crystal structure of adenylyl cyclase (AC) suggest the existence of
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