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Merck

F1283

Sigma-Aldrich

Fetal Bovine Serum

Fetal Bovine Serum, USA origin, Ultra Low IgG

别名:

FBS, FCS, Fetal Calf Serum

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

生物源

bovine fetus

品質等級

無菌

sterile-filtered

產品線

FBS Select

成份

Bovine IgG, <5 μg/mL
Hemoglobin, ≤20 mg/dL

來源

USA origin

儲存條件

avoid repeated freeze/thaw cycles
protect from light

技術

cell culture | mammalian: suitable

雜質

≤10 EU/mL endotoxin

pH值

6.8-8.1

滲透度

260-330 mOsm/kg H2O

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.

Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.

IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.

包裝

Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.

準備報告

The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.

Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.

分析報告

Endotoxin and hemoglobin tested

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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