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Merck

E2264

Sigma-Aldrich

内切糖苷酶F3 from Elizabethkingia miricola

recombinant, expressed in E. coli, 30 U/mg

别名:

内切-β-N-乙酰氨基葡萄糖苷酶 F3, 内切糖苷酶F3 来源于脑膜脓毒性伊丽莎白菌(金黄杆菌属/黄质菌属), 微小伊丽莎白菌

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About This Item

MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

共軛

(N-linked)

形狀

solution

比活性

30 U/mg

分子量

32 kDa

運輸包裝

wet ice

儲存溫度

2-8°C

應用

来自 和平空间站伊金氏菌 的内切糖苷酶 F3 已用于分析血清蛋白的核心岩藻糖基化和胰蛋白酶消化物。

生化/生理作用

内切糖苷酶 F3 属于糖苷水解酶家族 18 (GH18)。具有水解活性。内切糖苷酶 F3 糖基化 α-1,6-岩藻糖基化 GlcNAc 衍生物,得到天然核心岩藻糖基化复合物型 N -糖肽。
裂解天冬酰胺连接的双天线和三天线复合物,寡糖取决于核心岩藻糖基化和肽键的状态。

包裝

提供 5× 反应缓冲液,250 mM 醋酸钠,pH 4.5

單位定義

一个单元将在37°C pH 4.5 下每分钟从 1μmole 变性猪纤维蛋白原释放 N-连接寡糖。

外觀

20 mM Tris-HCl,pH 7.5中的无菌填充溶液

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Advances in Carbohydrate Chemistry (2016)
Chemical Biology of Glycoproteins (2017)
Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM
Ma J, et al.
Journal of proteomics, 189, 67-74 (2018)
Characterization of novel endo-beta-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG
Huang Y, et al.
Scientific reports, 8(1), 246-246 (2018)
Liwei Cao et al.
Nature communications, 13(1), 3910-3910 (2022-07-08)
Core fucosylation of N-linked glycoproteins has been linked to the functions of glycoproteins in physiological and pathological processes. However, quantitative characterization of core fucosylation remains challenging due to the complexity and heterogeneity of N-linked glycosylation. Here we report a mass

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Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

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