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E0639

Sigma-Aldrich

内切糖苷酶F2 from Elizabethkingia miricola

recombinant, expressed in E. coli, 20 U/mg

别名:

内-β-N-乙酰氨基葡糖苷酶F2，Endo F2, 内切糖苷酶F2 来源于脑膜炎脓毒性黄杆菌, 内切糖苷酶F2 来源于脑膜脓毒性伊丽莎白菌, 内切糖苷酶F2 来源于脑膜脓毒性金黄杆菌, 米尔伊丽莎白菌

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About This Item

CAS号:
37278-88-9
EC 号:
3.2.1.96 (BRENDA, IUBMB)
MDL號碼:
MFCD00151069
分類程式碼代碼:
12352204
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

200

共軛

(N-linked)

形狀

solution

比活性

20 U/mg

分子量

32 kDa

運輸包裝

wet ice

儲存溫度

2-8°C

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包裝

提供 5× 反应缓冲液，250 mM 醋酸钠，pH 4.5

單位定義

一个单元将在37 °C、pH 4.5下每分钟从1 μmole变性猪纤维蛋白原释放N-连接寡糖。

外觀

无菌灌装溶液，含10 mM乙酸钠、25 mM氯化钠，pH 4.5

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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糖苷酶F 来源于脑膜脓毒性伊丽莎白菌 BioReagent, ≥95% (SDS-PAGE), for proteomics

Sigma-Aldrich

P7367

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

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It has been demonstrated previously that Enterococcus faecalis produces secreted endoglycosidases that enable the bacteria to remove N-linked glycans from glycoproteins. One enzyme potentially responsible for this activity is EF0114, comprising a typical GH18 endoglycosidase domain and a GH20 domain.
Helena Ryšlavá et al.
The FEBS journal, 278(14), 2469-2484 (2011-05-14)
Fungal β-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. It has high GalNAcase activity, tolerates substrates with the modified N-acyl group
M V Padkina et al.
Prikladnaia biokhimiia i mikrobiologiia, 46(4), 448-455 (2010-09-29)
The HuIFNA16, HuIFNB, and BoIFNG genes encoding human [alpha]16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed. There was no
Valentina Botti et al.
PloS one, 6(8), e23838-e23838 (2011-08-23)
Hepatitis C Virus E1E2 heterodimers are components of the viral spike. Although there is a general agreement on the necessity of the co-expression of both E1 and E2 on a single coding unit for their productive folding and assembly, in
Roger S Zou et al.
Aging, 3(10), 968-984 (2011-10-13)
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form
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