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形狀
liquid
用途
sufficient for 250 reactions
特點
dNTPs included
hotstart: no
濃度
1 unit/μL
技術
PCR: suitable
顏色
red
輸入
purified DNA
適合性
suitable for PCR
運輸包裝
wet ice
儲存溫度
−20°C
相关类别
一般說明
REDTaq® SuperPak™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.
應用
REDTaq® SuperPak™ DNA Polymerase has been used in:
- reverse transcriptase-polymerase chain reaction (RT-PCR)
- nested PCR
- routine PCR amplification
特點和優勢
- Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
- Suitable for nested PCR
- Contains ultrapure 10mM dNTP mix in a separate vial
法律資訊
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
D Alvira et al.
Neuroscience, 147(3), 746-756 (2007-06-23)
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A Jóźwik et al.
Veterinary research communications, 29(4), 347-359 (2005-03-09)
Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
M Yeste-Velasco et al.
Neuroscience, 159(3), 1135-1147 (2009-04-10)
Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
M Yeste-Velasco et al.
Neuropharmacology, 53(2), 295-307 (2007-07-07)
Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
实验方案
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
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