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Merck

D2197

Sigma-Aldrich

心肌黄酶 来源于克鲁氏梭状芽孢

recombinant, expressed in E. coli, Animal-component free

别名:

心肌黄酶, 硫辛酰胺脱氢酶, 硫辛酰脱氢酶

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

形狀

solid

比活性

>=30 units/mg protein (biuret)

儲存溫度

−20°C

一般說明

Research area: Cancer

應用

克鲁维氏梭菌的心肌黄酶已用于鳃细胞中的乳酸脱氢酶测定,并浸渍在条带上进行甲酸脱氢酶测定。

生化/生理作用

心肌黄酶在电子受体(例如亚甲基蓝或2,6-二氯苯酚吲哚酚)存在下催化β-NADH或β-NADPH的氧化。 已知对β-NADH或β-NADPH具有特异性的心肌黄酶。Straub的猪心脏酶可能具有天然心肌黄酶(β-NADH特异性)以及硫辛酸和硫辛酰胺脱氢酶活性。据报道它是一种单体蛋白质。然而,Massey报道心肌黄酶可能是一种变性的脂硫辛酰胺脱氢酶。将猪心脏制剂与Cu2+进行预孵育,可降低硫辛酰胺脱氢酶活性并呈比例增加β-NADH心肌黄酶活性。在我们的实验室中,我们已经在一定程度上证明了这种对猪心脏酶的铜效应,但在克氏梭菌 或圆酵母制剂中没有观察到明显的效果。硫辛酰胺脱氢酶:心肌黄酶的比率可作为一种变性的量度。

單位定義

一个单位的心肌黄酶将在25℃、pH 7.5下每分钟氧化1.0微摩尔的β-NADH,并相应还原适当的电子受体。

外觀

以含有海藻糖冻干粉形式提供。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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