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Merck

CS0410

Sigma-Aldrich

谷胱甘肽S-转移酶(GST)活性检测试剂盒

sufficient for 500 multiwell tests

别名:

GST Enzyme Activity Kit

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 500 multiwell tests

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

一般說明

谷胱甘肽S-转移酶 (GST) 检测试剂盒用于测定总GST的活性。适用于细胞和细菌裂解物、组织匀浆以及血浆
和红细胞裂解物样品。

應用

该谷胱甘肽S-转移酶检测试剂盒采用1-氯-2,4-二硝基苯(CDNB),适用于广泛的GST同工酶。基本原理是谷胱甘肽硫醇基团结合到CDNB底物上后,在340nm处的吸光度会增加。

生化/生理作用

谷胱甘肽S-转移酶 (GST)是在哺乳动物解除许多外源物毒素过程中发挥重要作用的一组酶。该酶可将谷胱甘肽的硫醇基团连接到亲电的外源物上,保护细胞免受毒物损害,进一步帮助细胞抵抗化合物诱变、致癌和毒性作用。GST活性广泛存在于植物、昆虫、酵母、细菌以及大多数哺乳动物组织中,尤其是在解毒过程中发挥关键作用的肝脏中。

仅试剂盒组分

产品编号
说明

  • Dulbecco's Phosphate Buffered Saline 100 mL

  • L-Glutathione Reduced 1 g

  • GST (Control) .1 mL

  • Sample Buffer 5 mL

  • Substrate (CDNB) 1.2 mL

訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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