HTC

93120108, rat liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: HTC, 93120108
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: 2n = 66, hypertriploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Dexamethasone phosphate; hydrocortisone
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat hepatoma

細胞系描述

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

應用

Tumourigenicity and enzymatic studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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