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Merck
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Key Documents

AR42J

93100618, rat pancreas, Pancreas cells

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About This Item

分類程式碼代碼:
41106514

product name

AR42J, 93100618

生物源

rat pancreas

增長模式

Semi-adherent aggregates

染色體組型

Not specified

形態學

Pancreas cells

產品

Amylase and exocrine enzymes

受體

Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Rat exocrine pancreatic tumour

細胞系描述

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

應用

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

其他說明

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