推荐产品
product name
AR42J, 93100618
生物源
rat pancreas
增長模式
Semi-adherent aggregates
染色體組型
Not specified
形態學
Pancreas cells
產品
Amylase and exocrine enzymes
受體
Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat exocrine pancreatic tumour
細胞系描述
Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.
應用
AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex
其他說明
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