GS-109-V-20

90110505, human skin, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• product name: GS-109-V-20, 90110505
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian skin fibroblast Gardner′s syndrome

細胞系描述

Derived from skin from the upper arm of an 18 year old Caucasian male with Gardner′s syndrome is an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. Cells exist in an initiated state rather than a normal or transformed state. Normal diploid karyotype.

應用

Tumourigenicity studies

培養基

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Monolayer will form in a 25cm² flask in 1 week.

其他說明

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