MNNG/HOS (TE85, Clone F-5)

87070201, human bone, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: MNNG/HOS (TE85, Clone F-5), 87070201
• 生物源: human bone
• 增長模式: Adherent
• 染色體組型: Modal no. 70, 71; hypertriploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian osteosarcoma, chemically transformed

細胞系描述

A chemically transformed cell line (MNNG 0.01ug/ml) derived from an osteosarcoma of a 13 year female Caucasian. Grows to high saturation density and produces tumours in nude mice.

應用

Transformation and tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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