WI 38VA13 Subline 2RA

85062512, human lung (fetal), Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: WI 38VA13 Subline 2RA, 85062512
• 生物源: human lung (fetal)
• 增長模式: Adherent
• 染色體組型: 2n = 46, hyperdiploid, modal no. 73-78
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian foetal lung, SV40 transformed

細胞系描述

An SV40 transformed derivative of WI 38. The cells are characterised by loss of contact inhibition, unlimited proliferation and the presence of SV40 antigens.

應用

Transformation and virus studies: SV40

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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