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Key Documents

C-27020

Sigma-Aldrich

Osteoblast Mineralization Medium

Ready-to-use kit including Basal Medium and SupplementMix, 100 ml

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.81

形狀

liquid

技術

cell culture | mammalian: suitable

運輸包裝

wet ice

一般說明

Osteoblast Mineralization Medium (Ready-to-use)

應用

Osteoblast Mineralization Medium has been developed for the efficient induction of strong mineralization in human osteoblast cultures. Extensive extracellular calcium-deposits can be observed after 21 days by following an easy one-step protocol. The medium is optimized for primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines.Recommended for:Human Osteoblasts (HOB)

品質

All lots of PromoCell Osteoblast Mineralization Medium are tested for their capacity to induce mineralization in primary human osteoblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

準備報告

Contains all the growth factors and supplements necessary for mineralization of human osteoblasts.

儲存和穩定性

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

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儲存類別代碼

10 - Combustible liquids


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商品

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

Primary human osteoblasts and media for the optimal growth and mineralization of human osteoblast cultures. Protocols for handling, cell passaging, media, and product use.

相关内容

Cell culture protocol used to differentiate primary human osteoblasts into bone cells in 2-3 weeks.

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