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Key Documents

C-22162

Sigma-Aldrich

Smooth Muscle Cell Growth Medium 2

Kit including Basal Medium and SupplementPack, 500 ml

别名:

Primary Cell Culture Media

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

形狀

liquid

包裝

pkg of 500 ml

技術

cell culture | mammalian: suitable

運輸包裝

wet ice

一般說明

Smooth Muscle Cell Growth Medium 2 Kit

應用

Smooth Muscle Cell Growth Medium 2 is a low-serum (5% V/V) medium developed to establish and maintain smooth muscle cell cultures from blood vessels and hollow organs. The medium is optimized for primary human cells but can also be used for bovine, porcine, murine, dog, and rat smooth muscle cells.Recommended for:Human Umbilical Artery Smooth Muscle Cells (HUASMC)Human Coronary Artery Smooth Muscle Cells (HCASMC)Human Pulmonary Artery Smooth Muscle Cells (HPASMC)Human Aortic Smooth Muscle Cells (HAoSMC)Human Bronchial Smooth Muscle Cells (HBSMC)Human Tracheal Smooth Muscle Cells (HTSMC)Human Uterine Smooth Muscle Cells (HUtSMC)

成分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Smooth Muscle Cell Growth Medium are subjected to comprehensive quality control tests using primary human smooth muscle cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested smooth muscle cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

準備報告

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 0.5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 2 ng / mlInsulin (recombinant human): 5 µg / ml

儲存和穩定性

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

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儲存類別代碼

10 - Combustible liquids


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Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

Smooth muscle human primary cells isolated from blood vessels and hollow organs, plus smooth muscle cell growth media. Protocols on media handling, cell passaging and product use.

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