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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
CY5-15, monoclonal
形狀
buffered aqueous solution
包裝
antibody small pack of 25 μL
濃度
~1.5 mg/mL
技術
direct ELISA: suitable
dot blot: 1-2 μg/mL using cell protein extracts labeled with Cy5
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
同型
IgG1
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
一般說明
CyDyes属于荧光团家族,可用于标记蛋白质、肽、DNA、RNA和其他生物分子。这些染料是小分子,对pH不敏感(在pH 3-10之间),可溶于水溶液,并且耐受DMSO。它们比荧光素更稳定,具有高摩尔消光系数和良好的量子产率。它们的物理和化学性质使其(主要是Cy3和Cy5)应用于许多不同的生物测定,例如:DNA微阵列,蛋白质微阵列,二维蛋白质分析(2D凝胶),荧光共振能量转移(FRET)和免疫组化分析。
小鼠单克隆克隆CY5-15抗Cy5抗体可识别Cy5和AlexaFluor647标记蛋白,但不识别Cy3标记蛋白。
特異性
Cy5单克隆抗体可识别Cy5和AlexaFluor647标记蛋白,但不识别Cy3标记蛋白。
该抗体可识别Cy5和AlexaFluor 647标记蛋白,但不识别Cy3标记蛋白。
免疫原
Cy5标记蛋白的混合物。
應用
小鼠单克隆克隆CY5-15抗Cy5抗体被用于标记CyDye5,用于通过免疫细胞化学和免疫组织化学(IHC)技术(如ELISA、免疫沉淀、免疫细胞化学、斑点印迹测定和蛋白质微阵列)进行检测、定量和信号放大。在使用Cy5标记生物分子的测定中,Cy5抗体是Cy5信号放大的重要工具。
小鼠抗Cy5单克隆抗体已用于免疫荧光测定和蛋白质免疫印迹。
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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