推荐产品
生物源
bovine
等級
for molecular biology
產品線
BioReagent
化驗
≥98%
形狀
lyophilized powder
分子量
~66 kDa
純化經由
heat shock fractionation
包裝
poly bottle of
來源
USA origin
技術
ELISA: suitable
electrophoresis: suitable
western blot: suitable
pH值
7
溶解度
water: soluble (40 mg/ml)
UniProt登錄號
異物活動
Protease free
儲存溫度
2-8°C
基因資訊
bovine ... ALB(280717)
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應用
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.
生化/生理作用
BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。
特點和優勢
- Protease free
- Suitable for blocking non-specific protein binding sites in hybridizations
- Heat shock fractionated
準備報告
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
采用热休克分级分离法制备
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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实验方案
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
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