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Merck

A3688

Sigma-Aldrich

抗-小鼠 IgG(全分子)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

别名:

Goat Anti-Mouse IgG (whole molecule)−AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

mouse

應無反應活性

human

技術

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
山羊抗小鼠IgG(全分子)-碱性磷酸酶抗体使用IEP与正常小鼠血清和小鼠IgG反应。通过ODD法,抗血清与小鼠IgG1、IgG2a、IgG2b、IgG3、IgA和IgM反应。通过ELISA,偶联物显示与人血清蛋白无反应性。

免疫原

纯化小鼠IgG

應用

使用碱性磷酸酶偶联的山羊抗小鼠IgG作为次级抗体,在37℃下1小时,通过蛋白质印迹分析人和鼠肿瘤细胞裂解液的各种蛋白表达。
在含有终浓度为0.5M NaCl的TBS/Tween中,使用碱性磷酸酶缀合的山羊抗小鼠IgG(μg/ ml)作为二抗,对支气管肺泡液中的表面活性蛋白A进行检测。
山羊抗小鼠IgG(全分子)-碱性磷酸酶抗体可用于免疫细胞化学分析和ELISA。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1篇论文)

其他說明

与人血清蛋白吸附的抗体。

外觀

在0.05 M Tris(pH 8.0)中的溶液,含1%牛血清白蛋白、10 mM甘氨酸、1 mM MgCl2、50%甘油和15 mM叠氮化钠。

準備報告

经过吸附以减少人体样本的背景染色。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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