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Merck

01673

Sigma-Aldrich

吖啶黄

suitable for fluorescence, BioReagent, ≥90% (AT)

别名:

中性吖啶黄素, 优黄素, 氯代3,6-二氨基-10-甲基吖啶和3,6-二氨基吖啶(原黄素)混合物

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About This Item

经验公式(希尔记法):
C14H14ClN3
CAS号:
分子量:
259.73
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.32

產品線

BioReagent

化驗

≥90% (AT)

形狀

powder

成份

Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC

溶解度

H2O: 0.33 g/mL (lit.)(lit.)

螢光

λex 463 nm; λem 490 nm in ethanol

適合性

suitable for fluorescence

SMILES 字串

[Cl-].Nc1ccc2cc3ccc(N)cc3nc2c1.C[n+]4c5cc(N)ccc5cc6ccc(N)cc46

InChI

1S/C14H13N3.C13H11N3.ClH/c1-17-13-7-11(15)4-2-9(13)6-10-3-5-12(16)8-14(10)17;14-10-3-1-8-5-9-2-4-11(15)7-13(9)16-12(8)6-10;/h2-8H,1H3,(H3,15,16);1-7H,14-15H2;1H

InChI 密鑰

PEJLNXHANOHNSU-UHFFFAOYSA-N

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應用

吖啶黄用于荧光标记高分子量 RNA 分子 。
各种溶剂中的激发和发射波长 :
  • 甲醇:λex = 424 nm;λem = 518 nm
  • 乙醇:λex = 426 nm;λem = 524 nm
  • 丙醇:λex = 430 nm;λem = 512 nm
  • 丁醇:λex = 430 nm;λem = 526 nm
  • 甲酰胺:λex = 434 nm;λem = 524 nm
  • 甘油:λex = 432 nm;λem = 540 nm
  • 水:λex = 416 nm;λem = 514 nm

不溶于乙醚、氯仿和固定油。在荧光稳态测量中用作供体分子(当与罗丹明 6G 作为受体配对时),起到 pH 传感器的作用 。

其他說明

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously

象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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