跳转至内容
Merck
所有图片(2)

Key Documents

MABE340-AF555

Sigma-Aldrich

Anti-MDM2 Antibody, clone IF2, Alexa Fluor 555 Conjugate

clone IF2, from mouse, ALEXA FLUOR 555

别名:

E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.45

生物源

mouse

品質等級

共軛

ALEXA FLUOR 555

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

IF2, monoclonal

物種活性

human

技術

immunocytochemistry: suitable

同型

IgG2b

NCBI登錄號

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

human ... MDM2(4193)

一般說明

E3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.

特異性

Clone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).

免疫原

Epitope: Within amino acids 26-169.
Recombinant human MDM2.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in HeLa cells.
The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Apoptosis - Additional
This Anti-MDM2 Antibody, clone IF2, Alexa Fluor 555 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.

品質

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HepG2 cells.

標靶描述

~85 kDa observed.

外觀

Protein G purified.
Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门