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生物源
mouse
品質等級
共軛
ALEXA FLUOR™ 555
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
IF2, monoclonal
物種活性
human
技術
immunocytochemistry: suitable
同型
IgG2b
NCBI登錄號
UniProt登錄號
目標翻譯後修改
unmodified
基因資訊
human ... MDM2(4193)
一般說明
E3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.
特異性
Clone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).
免疫原
Epitope: Within amino acids 26-169.
Recombinant human MDM2.
應用
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in HeLa cells.
The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-MDM2 Antibody, clone IF2, Alexa Fluor™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.
品質
Evaluated by Immunocytochemistry in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HepG2 cells.
標靶描述
~85 kDa observed.
外觀
Protein G purified.
Purified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
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