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Key Documents

MAB4037

Sigma-Aldrich

Anti-PTEN Antibody, CT, clone A2b1

clone A2b1, Chemicon®, from mouse

别名:

MMAC1, TEP1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

A2b1, monoclonal

物種活性

human, rat, mouse

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1

適合性

not suitable for immunohistochemistry (Paraffin)

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

特異性

Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.

*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.

免疫原

Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus

應用

Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)

Immunocytochemistry

Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.

Immunoprecipitation 1:10 to 1:20

Protocol for immunohistochemistry of frozen tissue sections on glass slides.

1. Fix sections for 5 minutes with cold acetone.

2. Air dry

3. Wash sections with PBS

4. Add blocking solution (horse serum) for 20 minutes

5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.

6. Wash with PBS

7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).

8. Incubate for 30 minutes at room temperature.

9. Wash with PBS

10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.

11. Wash with PBS

12. Develop with DAB substrate.

13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.

標靶描述

55 kDa

聯結

Replaces: 04-409

外觀

Protein A purified
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

儲存和穩定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析報告

Control
Breast tumor tissue

Purified active kinase is Catalogue Number 14-488

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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