推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
monkey, mouse, human, bovine
製造商/商標名
Chemicon®
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... APP(351)
特異性
淀粉样前体蛋白(APP),KPI结构域。
免疫原
APP。
應用
淀粉样前体蛋白抗体(KPI结构域)是一种针对淀粉样前体蛋白的抗体,可用于IP、WB、IC、IH。
蛋白质印迹:1:250 - 1:1,000
免疫沉淀:需要对二硫键进行还原和烷基化。
免疫细胞化学:对于Cos7和NteraZ细胞系,需要对二硫键进行还原和烷基化。
免疫组化:用于甲醛固定、石蜡包埋的组织时,需要对二硫键进行还原和烷基化或微波。
最佳工作稀释度必须由最终用户进行确定。
还原/烷基化方案:
在黑暗中将载玻片(印迹)在TBS(PBS)+ 50mM 2-巯基乙醇中37°C孵育3小时进行还原。用TBS洗涤两次,然后在TBS(PBS)+ 20 mg/ml碘乙酸中室温孵育30 min。用TBS(PBS)洗涤两次,然后正常封闭和处理。
免疫沉淀:需要对二硫键进行还原和烷基化。
免疫细胞化学:对于Cos7和NteraZ细胞系,需要对二硫键进行还原和烷基化。
免疫组化:用于甲醛固定、石蜡包埋的组织时,需要对二硫键进行还原和烷基化或微波。
最佳工作稀释度必须由最终用户进行确定。
还原/烷基化方案:
在黑暗中将载玻片(印迹)在TBS(PBS)+ 50mM 2-巯基乙醇中37°C孵育3小时进行还原。用TBS洗涤两次,然后在TBS(PBS)+ 20 mg/ml碘乙酸中室温孵育30 min。用TBS(PBS)洗涤两次,然后正常封闭和处理。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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