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Merck
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文件

AB2272

Sigma-Aldrich

抗ZO -1抗体

from rabbit, purified by affinity chromatography

别名:

tight junction protein 1, zonula occludens 1 protein, Zona occludens protein 1, Zonula occludens protein 1, zona occludens 1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

100

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

物種活性（以同源性預測）

mouse (based on 100% sequence homology)

包裝

antibody small pack of 25 μg

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI登錄號

NP_003248

UniProt登錄號

Q07157

運輸包裝

ambient
wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... TJP1(7082)

一般說明

紧密连接是一种蛋白质复合物，其在上皮细胞和内皮细胞的质膜结构域之间产生细胞间边界。许多紧密连接相关蛋白是膜相关鸟苷酸激酶（MAGUK）家族的成员，包括闭合蛋白和紧密连接蛋白家族ZO-1、ZO-2和ZO-3。这些蛋白被认为具有结构和信号功能，并由三个蛋白-蛋白相互作用模块来特征：PDZ结构域、SH3结构域和鸟苷酸激酶（GuK）结构域。ZO-1可与ZO-2或ZO-3形成复合物。此外，这些蛋白还可以在紧密连接之上与闭合蛋白、咬合蛋白和F-肌动蛋白结合，在它们之间提供肌动蛋白细胞骨架与紧密连接之间的联系。在许多乳腺癌细胞系中，ZO-1表达均明显降低。ZO-2和ZO-3在上皮紧密连接内普遍表达，而ZO-1则在心肌细胞的细胞连接处表达，ZO-2在非上皮组织中不表达。

特異性

其他同源性：大鼠（89％序列同源性）。

免疫原

对应于人ZO-1的KLH缀合的线性肽。

應用

使用经过验证可用于WB、IH（P）的抗ZO-1抗体对ZO-1进行检测。
免疫组化分析： 一个先前批次产品以1：500的稀释度在正常人睾丸组织中检测到ZO-1。
研究子类别
神经 & 胶质标记
研究类别
神经科学

品質

通过蛋白质印迹对HepG2细胞裂解液进行了评估。

蛋白质印迹分析：0.1 µg/ml该抗体可在10 µg的HepG2细胞裂解液中检测到ZO-1。

標靶描述

观测值〜260 kDa

外觀

亲和纯化
在含 0.1 M Tris-甘氨酸（pH 7.4），150 mM NaCl 和 0.05％ 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

儲存和穩定性

自发运之日起，在 2-8°C 条件下可稳定保存1年

分析報告

对照
HepG2 细胞裂解液

其他說明

浓度：请参考批次特异性浓缩物的分析证书。
浓度：请参考特定批次的数据表。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Mikolaj Adamek et al.
Virulence, 12(1), 1855-1883 (2021-07-17)
Gills of fish are involved in respiration, excretion and osmoregulation. Due to numerous interactions between these processes, branchial diseases have serious implications on fish health. Here, "koi sleepy disease" (KSD), caused by carp edema virus (CEV) infection was used to
Sung-Eun Choi et al.
Experimental and therapeutic medicine, 24(5), 648-648 (2022-09-29)
Tubulointerstitial fibrosis is characterized by accumulation of the extracellular matrix in the interstitium. Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase family, is known for promoting cancer metastasis, invasion and stromal fibrosis in various organs. Our previous study
Dina Gadalla et al.
Journal of biomedical materials research. Part A, 110(6), 1251-1262 (2022-02-11)
There are reciprocal interactions between epithelial cells and underlying basement membrane. The resemblance of biomaterials to native basement membrane is thus critical for their success when used to regenerate epithelium-containing organs. Particularly, the use of nanofibers and the incorporation of
Kepeng Ou et al.
Journal of cellular and molecular medicine, 24(7), 3958-3970 (2020-03-07)
Diabetic retinopathy (DR) is one of the most severe clinical manifestations of diabetes mellitus and a major cause of blindness. DR is principally a microvascular disease, although the pathogenesis also involves metabolic reactive intermediates which induce neuronal and glial activation
Artem V Fedorov et al.
Cells, 11(21) (2022-11-12)
Mitochondria-targeted antioxidants have become promising candidates for the therapy of various pathologies. The mitochondria-targeted antioxidant SkQ1, which is a derivative of plastoquinone, has been successfully used in preclinical studies for the treatment of cardiovascular and renal diseases, and has demonstrated
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