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Merck
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文件

69671

Millipore

凝血酶，限制级

别名:

凝血酶，限制级

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About This Item

CAS号:
9002-04-4
分類程式碼代碼:
12352202
NACRES:
NA.54

生物源

human plasma

品質等級

400

形狀

liquid

製造商／商標名

Novagen®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

技術

protein extraction: suitable

適合性

suitable for additive or modifier in the separation of proteins or peptides

應用

life science and biopharma

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

限制级凝血酶是一种高度纯化的制剂，能够特异性切割用适当载体产生的靶蛋白。该制剂通过融合蛋白活性功能测试，不含可检测的污染蛋白酶。由经认证测试对HBsAg以及HIV和HCV抗体阴性的人血浆制制备而成。凝血酶以50%甘油溶液的形式提供，包括10倍凝血酶裂解缓冲液和裂解控制蛋白。

成分

•50 U凝血酶

•1 ml10X 凝血酶裂解缓冲液

•2 ml1X 凝血酶稀释/储存缓冲液

•10 µg裂解对照蛋白

警告

毒性：多个毒性值，请参阅MSDS（O）

單位定義

一个单位定义为：在200 µl反应中，在20°C下，在16小时内裂解1 mg融合蛋白所需的酶量，该反应含有20 mM Tris-HCl pH 8.4、150 mM NaCl、2.5 mM CaCl₂、50 µg融合蛋白。

準備報告

由经认证测试对HBsAg以及HIV和HCV抗体阴性的人血浆制制备而成。

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

分析证书(COA)

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Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one

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