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product name
百日咳毒素，无盐，百日咳杆菌, This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.
品質等級
形狀
lyophilized
製造商／商標名
Calbiochem®
儲存條件
do not freeze
異物活動
adenylate cyclase ≤35 pmol/min-μg (in the presence of calmodulin)
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
Pertussis toxin (PT), secreted protein exotoxin and virulence factor by Bordetella pertussis, is the causative agent of whooping cough. It is transported across the bacterial outer membrane through a type IV secretion system encoded by the ptl genes, situated downstream from the ptx genes that encode the toxin. This complex multisubunit toxin with an AB5 configuration, serves as a crucial component of acellular pertussis vaccines. It can function protectively as a single-component toxoid vaccine. Moreover, it is widely used in mammalian cell signaling studies due to its inhibitory effect on G-protein-coupled receptor pathways. It inhibits neutrophil recruitment and airway influx, a common inflammatory response to bacterial infection.
應用
Pertussis Toxin, Bordetella pertussis has been used as a Gi protein inhibitor to study its ability to reduce the depolarizing responses of hypothalamic pro-opiomelanocortin (POMC) neurons. It has also been used in pertussis toxin experiments to block Gαi for an in vivo inner ear and an in vitro outer hair cell (HC) study.
生化／生理作用
主要靶标
鸟嘌呤核苷酸结合调节蛋白Gi，Go和Gt
鸟嘌呤核苷酸结合调节蛋白Gi，Go和Gt
产物不与ATP竞争。
警告
毒性：有毒（F）
外觀
在50 mM NaCl，10 mM磷酸钠缓冲液，pH 7.0中冻干。
重構
在复溶后，冷藏（4°C）。为了在溶液中长期保存，请使用高离子强度缓冲液（例如100 mM磷酸钠，500 mM氯化钠，pH值7.0）复溶。贮备液在CHO细胞试验中可稳定至少6个月，在非细胞系统中可稳定1-2个月。
用500µl H₂O复溶。每次使用前，刮擦管壁并轻轻移取溶液混合，以获得均匀的混悬剂。
分析報告
SDS-PAGE法测得5个不同条带
其他說明
Joshi, S.A., et al. 1998.FEBS Lett. 441, 67.
Fryer, M.W.1992.Neurosci. Lett.146, 84.
Gierschik, P. 1992.Curr.Topics Microbiol.Immunol.175, 69.
Harada, J., et al. 1992.Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L.1992.J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992.FASEB J.6, 2684.
Zhu, J., et al. 1992.J. Pharmacol.Exp.Therap.263, 1479.
Hewlett, E.L., et al. 1983.Infect.Immun.40, 1198.
Moss, J., et al. 1983.J. Biol. Chem.258, 11879.
Fryer, M.W.1992.Neurosci. Lett.146, 84.
Gierschik, P. 1992.Curr.Topics Microbiol.Immunol.175, 69.
Harada, J., et al. 1992.Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L.1992.J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992.FASEB J.6, 2684.
Zhu, J., et al. 1992.J. Pharmacol.Exp.Therap.263, 1479.
Hewlett, E.L., et al. 1983.Infect.Immun.40, 1198.
Moss, J., et al. 1983.J. Biol. Chem.258, 11879.
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法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Pertussis Toxin: Structure-Function Relationship
Locht C, et al.
Bacterial Protein Toxins, 167-185 (2000)
Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2
Tona Y and Wu DK
eLife, 9, e59282-e59282 (2020)
Nicholas H Carbonetti
Future microbiology, 5(3), 455-469 (2010-03-10)
Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two
P Órdenes et al.
Scientific reports, 11(1), 21644-21644 (2021-11-06)
Previous studies indicate that the activity of hypothalamic POMC neurons can be regulated by glucose via intracellular mechanisms, but its regulation by lactate is poorly understood. In addition to its energetic role, lactate acts as a signaling molecule. In this
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