391340

HEPES

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

• 别名: HEPES, 4-（2-羟乙基）哌嗪-1-乙磺酸, N-（2-羟乙基）哌嗪-N′-（2-乙磺酸）
• 经验公式（希尔记法）: C₈H₁₈N₂O₄S
• CAS号: 7365-45-9
• 分子量: 238.30
• Beilstein: 883043
• MDL號碼: MFCD00006158
• 分類程式碼代碼: 12161704
• NACRES: NA.25

属性

• product name: HEPES，游离酸，分子生物学级, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
• 等級: Molecular Biology
• 品質等級: 100
• 化驗: ≥99% (alkalimetric)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 顏色: white
• 有用的pH值範圍: 6.8-8.2
• pKa (25 °C): 7.5
• 溶解度: water: 400 mg/mL; aqueous base: soluble
• 正離子痕跡: heavy metals: ≤0.0005%
• 異物活動: DNase, RNase, and protease, none detected
• 運輸包裝: ambient
• 儲存溫度: 15-25°C
• SMILES 字串: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI 密鑰: JKMHFZQWWAIEOD-UHFFFAOYSA-N

说明

一般說明

两性离子N-取代的氨基磺酸缓冲液。在pH值为6.8-8.2的范围内有用。在 25°C 下的 pK_a 为7.48。

應用

• Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

警告

毒性：标准处理（A）

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable