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Merck
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Key Documents

234409

Sigma-Aldrich

Complement C9-Depleted Serum, Human

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.25

形狀

liquid

不包含

preservative

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

Human serum depleted of complement component C9 by affinity chromatography.
Human serum depleted of complement component C9 by affinity chromatography. C9-depleted serum, sterile-filtered through a 0.22 µm filter. No C9 detected by Ouchterlony using various dilutions of goat anti-human C9 antibody.

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

單位定義

Classical Pathway Activity: One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++.Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.

外觀

In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.

準備報告

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.

重構

Following initial thaw, aliquot and freeze (-70°C).

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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