23-023-M

SCF-Skp2 Protein Complex, Active, 10 µg

Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

• 别名: SCF ^Skp2, Skp2/Cks1 complex
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

属性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable
• NCBI登錄號: NM_001826; NM_003592; NM_005983; NM_014248; NM_170679
• 基因資訊: human ... Cks1(1163) , Cul1(8454) , Rbx1(9978) , Skp1(6500) , Skp2(6502)

说明

一般說明

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^Skp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition. Complex formation of SCF^Skp2 with Cks1 is required for the degradation of p27. An increased level of Skp2 and reduced level of p27 is linked to poor prognosis in a number of cancers

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany