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Merck
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Key Documents

217570

Sigma-Aldrich

抗-CD3小鼠mAb (UCHT1)

liquid, >95% (immunoglobulin, SDS-PAGE), clone UCHT1

别名:

Anti-CD3 Antibody, CD3 Detection Antibody, Mouse Anti-CD3

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

UCHT1, monoclonal

化驗

>95% (immunoglobulin, SDS-PAGE)

形狀

liquid

包含

0.5 mg/mL gentamycin sulfate as preservative

物種活性

human

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

同型

IgG1

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

抗CD3,小鼠单克隆,克隆UCHT1,可识别人外周T细胞中CD3复合物的ε-链。已针对冰冻切片的FC,IP,IHC和T细胞共刺激进行了验证。
蛋白A纯化的小鼠单克隆抗体。识别CD3 T细胞受体复合物的~20 kDaε链。
识别人外周T细胞中CD3复合物的ε-链。可用于激活表达CD3ε的T细胞。

免疫原

人胸腺细胞/Sezary T细胞

應用

流式细胞术(5 µg/ml)

冷冻切片(参见备注)

免疫沉淀法(参见备注)

T细胞共刺激(参见应用参考)

包裝

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

其他說明

Thibault, G. and Bardos, P. 1995.J. Immunol.154, 3814.
Beverley, P.C., and Callard, R.E.1981.Eur. J. Immunol.11, 329.
该抗体可用于激活表达CD3ε的T细胞(见应用参考)。对5×105培养的HPB-MLT人肿瘤细胞进行FACS。当与二级试剂山羊抗小鼠IgG(FITC偶联)一起使用时,测试的最佳结合浓度为5 µg/mL。据报道,该抗体可用于免疫沉淀和冷冻切片染色。合适的工作稀释度由与测定条件相关的变量决定。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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