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Key Documents

05-777

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, recombinant clone 4G10®

clone 4G10®, Upstate®, from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

4G10®, monoclonal

物種活性

vertebrates

製造商/商標名

Upstate®

技術

immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG2bκ

運輸包裝

wet ice

目標翻譯後修改

phosphorylation (pTyr)

一般說明

Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

免疫原

Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via Nickel affinity matrices.

應用

Anti-Phosphotyrosine Antibody, clone 4G10 is a recombinant antibody that detects tyrosine phosphorylated proteins in all species. This unique recombinant antibody is validated for use in IC, IH, IP, WB and is published in peer review journals.

品質

routinely evaluated by immunoblot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells

標靶描述

varies

外觀

Format: Purified
Protein G purified recombinant 4G10 mouse IgG2bk in 0.01M Phosphate Buffer containing no preservative, pH 7.1

分析報告

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

法律資訊

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Trond Methi et al.
European journal of immunology, 38(9), 2557-2563 (2008-09-16)
T cells with short interfering RNA-mediated Lck-knockdown (kd) display paradoxical hyper-responsiveness upon TCR ligation. We have previously reported a possible mechanism for T-cell activation in cells with low levels of Lck depending on Grb2-SOS1 recruitment to the zeta-chain of TCR/CD3
Sean Caenepeel et al.
Journal of experimental & clinical cancer research : CR, 29, 96-96 (2010-07-17)
Activating mutations in Kit receptor tyrosine kinase or the related platelet-derived growth factor receptor (PDGFR) play an important role in the pathogenesis of gastrointestinal stromal tumors (GIST). This study investigated the activity of motesanib, an inhibitor of vascular endothelial growth
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Scientific reports, 6, 28687-28687 (2016-07-02)
Monocyte chemotactic protein 1 (MCP1) stimulates phosphorylation of cortactin on Y421 and Y446 residues in a time-dependent manner and phosphorylation at Y446 but not Y421 residue is required for MCP1-induced CDK-interacting protein 1 (p21Cip1) nuclear export and degradation in facilitating
Sivareddy Kotla et al.
The Journal of biological chemistry, 290(51), 30306-30320 (2015-10-28)
Previously, we have demonstrated that 15(S)-hydroxyeicosatetranoic acid (15(S)-HETE) induces CD36 expression involving STAT1. Many studies have shown that peroxisome proliferator-activated receptor (PPAR)-γ mediates CD36 expression. Therefore, we asked the question whether these transcriptional factors interact with each other in the
Kazunobu Tachibana et al.
Molecular and cellular biology, 25(11), 4693-4702 (2005-05-19)
The development of the cardiovascular system and the development of the early hematopoietic systems are closely related, and both require signaling through the Tie2 receptor tyrosine kinase. Although endothelial cells and hematopoietic cells as well as their precursors share common

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